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Plot
Venus Versus Virus revolves around the life of the young girls named Sumire Takahana and Lucia Nahashi, who met as Sumire mysteriously discovered Lucia's secrets at the Venus Vanguard, a group who hunts demons called "Virus" lead by Lucia's father Nahashi. Now, while accepting jobs from people who find the Venus Vanguard brochure, they fight off the “Virus” while seeking their true roots and motives, and for that, Lucia will find that Sumire can be a lot more useful than she seems.
Main characters
 Sumire Takahana (?? ???, Takahana Sumire?)
Voiced by: Minori Chihara (Japanese), Natalie Arneson (English)
Sumire is a young high school girl and the newest member of the Venus Vanguard, despite being terribly scared of Viruses. While initially she cannot be of much help to Lucia, they eventually find out that whenever her body reacts to the special chemical used to defeat Viruses (the so called Vaccine), she transforms into a "Berserker Mode", or a living Anti-Virus. With this strange ability, she becomes stronger and violent, and is capable of defeating Viruses with her bare hands, though when under the influence of this power, she cannot discern friend from foe.
Lucia Nahashi (?? ???, Nahashi Ruchia?) 
Voiced by: Ayahi Takagaki (Japanese), Joanne Bonasso (English)
Lucia is a strong, young girl who lead the fights against Viruses alongside the other members of the Venus Vanguard, sworn to fight and kill all Viruses. Her left eye holds a mysterious powers, though is almost always covered with an eye patch. Use of her powers is taxing and wears her out, but can often be disastrous to her enemy in many ways. Her left arm has a symbol of the enchantment succeeded from her father.
S?ichir? Nahashi (?? ???, Nahashi S?ichir??)
Voiced by: J?r?ta Kosugi (Japanese), John Swasey (English)
S?ichir?, who is always referred to by his surname (as per Japanese respectful standard), is the guardian of Lucia who loves her as if he were her real father. Before Lucia was born, Nahashi was studying to become a mage along with Lucia's mother Lilith, and Lucia's father Lucif. He harbored an unrequited love for Lilith, but never got close with her as she was in love with Lucif.
Laura (???, R?ra?)
Voiced by: Ayumi Tsuji (Japanese), Kim-Ly Nguyen (English)
A mysterious blonde girl who lives with Lucia and Nahashi. She loves chocolate and is often seen eating it. She has a twin named Laila, and for some reason, neither of them seem to age. She is a major character right from the beginning in the anime, but in the manga she does not arrive right away.
Laila (???, Raira?)
Voiced by: Ayumi Tsuji
Laila is Laura's twin. After Lucif left to find the True World, Laila went with him and Laura stayed with Nahashi and Lilith. Laila loves confetti candy as much as Laura loves chocolate. Like Laura, she does not seem to age.
Lucif (????, Ryushifu?)
Voiced by: Daisuke Namikawa
Lucif is Lucia's father who, before she was born, left to find the path to the True World. His past is filled with mysteries, and Nahashi refuses to talk about the subject with Lucia.
Lilith (???, Ririsu?)
Voiced by: Wakana Yamazaki
Lilith is Lucia's mother who once worked under a mentor with Lucif and Nahashi to learn magic arts, but after he died, their training ceased. Lilith fell in love with Lucif and eventually became pregnant with Lucia, though by the time she was born, Lucif was already gone on his quest for the True World. A Virus allegedly killed Lilith when Lucia was still two years old.
Yoshiki (??, Yoshiki?)
Voiced by: Kiyotaka Furushima (Japanese), Blake Shepard (English)
Yoshiki is a young man Sumire met one day in a park while he was reading a book. After a few more meetings together while exchanging books to read, Yoshiki started to develop romantic feelings for Sumire
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